GRB7 Polyclonal antibody

GRB7 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10045-1-Ig

Synonyms

B47, GRB7, GRB7 adapter protein



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10045-1-Ig targets GRB7 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRB7 fusion protein Ag0023
Full Name growth factor receptor-bound protein 7
Calculated molecular weight 532 aa, 60 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC006535
Gene symbol GRB7
Gene ID (NCBI) 2886
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.

Protocols

Product Specific Protocols
WB protocol for GRB7 antibody 10045-1-IgDownload protocol
IHC protocol for GRB7 antibody 10045-1-IgDownload protocol
IF protocol for GRB7 antibody 10045-1-IgDownload protocol
IP protocol for GRB7 antibody 10045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols