GRB7 Polyclonal antibody

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• GRB7 Polyclonal antibody (10045-1-Ig)

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GRB7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10045-1-Ig Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Growth factor receptor-bound protein 7, GRB7 adapter protein, Epidermal growth factor receptor GRB-7, B47

• 

  WB analysis of A431 using 10045-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10045-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 10045-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 10045-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10045-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of A431 using 10045-1-Ig

  IP result of anti-GRB7 (IP:10045-1-Ig, 3ug; Detection:10045-1-Ig 1:500) with A431 cells lysate 3300ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 10045-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GRB7 antibody (10045-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10045-1-Ig targets GRB7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0023

  Product name: Recombinant human GRB7 protein

  Source: e coli.-derived, Unknow

  Tag: N/A

  Domain: Gst-KG-Grb7 of BC006535

  Sequence:

• Full Name: growth factor receptor-bound protein 7
• Calculated Molecular Weight: 532 aa, 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC006535
• Gene Symbol: GRB7
• Gene ID (NCBI): 2886
• RRID: AB_2232601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14451
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.

Publications

What published studies show

The GRB7 Polyclonal antibody (Cat# 10045-1-Ig) has 7 citations published in journals including Journal of Translational Medicine, Frontiers in Microbiology, Pharmaceuticals, International Immunopharmacology, Frontiers in Pharmacology, Molecular Carcinogenesis, and Bioengineered. The research fields span breast cancer progression, H. pylori-induced malignancy, ovarian cancer immune evasion, renal ischemia-reperfusion injury, myocardial ischemia/angiogenesis, colitis-associated colorectal cancer, and VEGF-mediated angiogenesis.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	J Transl Med; TCF12-regulated GRB7 facilitates the HER2+ breast cancer progression by activating Notch1 signaling pathway; Authors - Gang Wang
human	IHC,IF	Front Microbiol; GRB7-mediated enhancement of cell malignant characteristics induced by Helicobacter pylori infection; Authors - Huilin Zhao
human	WB	Pharmaceuticals (Basel); GRB7 Plays a Vital Role in Promoting the Progression and Mediating Immune Evasion of Ovarian Cancer; Authors - Liang Wen ; KO Validated
mouse,human	WB	Int Immunopharmacol; ACOT8-mediated palmitate accumulation promotes M1 macrophage polarization in renal ischemia-reperfusion injury via activation of the cGAS-STING pathway.; Authors - Haoxun Zhang
mouse	WB	Front Pharmacol; Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway; Authors - Mu-Xin Zhang
human,mouse	WB	Mol Carcinog; TRIM36 Inhibits the Development of AOM/DSS-Induced Colitis-Associated Colorectal Cancer by Promoting the Ubiquitination and Degradation of GRB7; Authors - Ju Wu