GRHPR Polyclonal antibody

GRHPR Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 51013-2-AP

Synonyms

GLXR, GLYD, GRHPR, PH2



Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, L02 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51013-2-AP targets GRHPR in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRHPR fusion protein Ag0484
Full Name glyoxylate reductase/hydroxypyruvate reductase
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000605
Gene symbol GRHPR
Gene ID (NCBI) 9380
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GRHPR(Glyoxylate reductase/hydroxypyruvate reductase) is also named as GLXR and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The GRHPR gene encodes a predicted 328-amino acid protein with a calculated molecular mass of 35.5 kD. It has glyoxylate reductase (GR), hydroxypyruvate reductase (HPR) and D-glycerate dehydrogenase (DGDH) activities(PMID:16597637). Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2)(PMID: 10484776). It can exsit as a homodimer(PMID:16756993).

Protocols

Product Specific Protocols
WB protocol for GRHPR antibody 51013-2-APDownload protocol
IHC protocol for GRHPR antibody 51013-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols