Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue, mouse cerebellum tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23350-1-AP targets GRIA4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19949 Product name: Recombinant human GRIA4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 87-229 aa of BC167786 Sequence: YSRGVFAIFGLYDKRSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRGALLSLLDHYEWNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFVIDCEIERLQNILEQIVSVG Predict reactive species |
| Full Name | glutamate receptor, ionotrophic, AMPA 4 |
| Calculated Molecular Weight | 902 aa, 101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC167786 |
| Gene Symbol | GRIA4 |
| Gene ID (NCBI) | 2893 |
| RRID | AB_2879262 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48058 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| IP protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| WB protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GRIA4 Polyclonal antibody (Cat# 23350-1-AP) has 15 citations across journals including Nature, Journal of Clinical Investigation, Cell Death & Disease, Molecular Psychiatry, Molecular Autism, Frontiers in Cell and Developmental Biology, Journal of Nutritional Biochemistry, iScience, Biochimica et Biophysica Acta, Journal of Neuroscience, Annals of Clinical and Translational Neurology, Molecular Brain, Journal of Psychiatric Research, and Thoracic Cancer. Research fields span neuroscience, epilepsy, Alzheimer's disease, autism spectrum disorder, Angelman syndrome, ALS-FTD, memory and synaptic function, and small cell lung cancer.
| Species | Application | Title |
|---|---|---|
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. | ||
Mol Psychiatry The dynamic impairment of synaptic transmission in the PCx-IL engram circuit contributes to early olfactory memory decline in Alzheimer's disease. | ||
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
Mol Autism UBE3A stabilization of β-catenin preserves synaptic proteins essential for motor and cognitive functions in Angelman Syndrome. |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody for Western Blot on cerebellar lysates at a 1:1000 dilution and reported that it works very well, producing reliable results with 20 µg of protein lysate. Overall, the feedback confirms strong performance and ease of use in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Andrea (Verified Customer) (07-24-2026) | It works very well by WB on cerebellar lysates (20ug of protein lysate)
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