GRIK2 Polyclonal antibody

GRIK2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13597-1-AP

Synonyms

EAA4, GLR6, GluK2, GluR 6, GLUR6, Glutamate receptor 6, GRIK2, MRT6

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Validation Data Gallery

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  WB analysis of human brain using 13597-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13597-1-AP (GRIK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 13597-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GRIK2 antibody (13597-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

13597-1-AP targets GRIK2 in WB, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRIK2 fusion protein Ag4516
• Full Name: glutamate receptor, ionotropic, kainate 2
• Calculated molecular weight: 908 aa, 103 kDa
• Observed molecular weight: 93-120 kDa
• GenBank accession number: BC037954
• Gene symbol: GRIK2
• Gene ID (NCBI): 2898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Mol Med; Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.; Authors - Sha Li