|Positive WB detected in||mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
13771-1-AP targets GPRIN1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GPRIN1 fusion protein Ag4754|
|Full Name||G protein regulated inducer of neurite outgrowth 1|
|Calculated molecular weight||1008 aa, 102 kDa|
|Observed molecular weight||100-110 kDa|
|GenBank accession number||BC052950|
|Gene ID (NCBI)||114787|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GPRIN1 (G protein-regulated inducer of neurite outgrowth 1), also named GRIN1, may be involved in neurite outgrowth. Western blotting analysis has confirmed its expression in mouse neuroblastoma and rat pheochromocytoma cells. And both GPRIN1 and GNAO1 are membrane-bound proteins that are enriched in the growth cones of neurites (PMID:10480904). It has 2 isoforms produced by alternative splicing. This antibody detects GRIN1 at bands around 110 kDa.
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