NMDAR1/GRIN1 Polyclonal antibody

• 

  WB analysis using 27676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 27676-1-AP

  IP result of anti-NMDAR1/GRIN1 (IP:27676-1-AP, 4ug; Detection:27676-1-AP 1:2000) with mouse brain tissue lysate 2100 ug.

• 

  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 27676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR1/GRIN1 antibody (27676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NMDAR1/GRIN1 antibody (27676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NMDAR1/GRIN1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Cat No. 27676-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Synonyms

GRIN1, NMDAR1, GluN1, Glutamate receptor ionotropic, NMDA 1, NMDAR 1

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF-Fro detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

27676-1-AP targets NMDAR1/GRIN1 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26364

  Product name: Recombinant human GRIN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 167-280 aa of NM_000832

  Sequence: MVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEAKELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGILGLQLINGKNESAH

• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_000832
• Gene Symbol: NMDAR1
• Gene ID (NCBI): 2902
• RRID: AB_2880944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05586
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
rat	WB	Aging (Albany NY); EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.; Authors - Zhao-Hui Yao
mouse	WB	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
mouse	WB	J Neurosci; Cortex-specific Tmem169 Deficiency Induces Defects in Cortical Neuron Development and Autism-like Behaviors in Mice; Authors - Junhao Wang
mouse	WB	Exp Neurol; DPP-4 inhibition by linagliptin ameliorates age-related mild cognitive impairment by regulating microglia polarization in mice; Authors - Fen Zhuge
rat	WB,IP	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang