Validation Data Gallery
|Positive WB detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
19953-1-AP targets NMDAR2A/GRIN2A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||glutamate receptor, ionotropic, N-methyl D-aspartate 2A|
|Calculated molecular weight||165 kDa|
|Observed molecular weight||150-160 kDa|
|GenBank accession number||NM_000833|
|Gene ID (NCBI)||2903|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRIN2A, also named as NMDAR2A and NR2A, belongs to the glutamate-gated ion channel (TC 1.A.10) family. GRIN2A is NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation of GRIN2A requires binding of agonist to both types of subunits. The antibody recognize the C-term of GRIN2A.
Biochim Biophys Acta
Specificity Protein 4 functionally regulates the transcription of NMDA receptor subunits GluN1, GluN2A, and GluN2B.
Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.
Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice.
Aging (Albany NY)
Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.
Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model.
J Mol Neurosci
Resveratrol Improves Synaptic Plasticity in Hypoxic-Ischemic Brain Injury in Neonatal Mice via Alleviating SIRT1/NF-κB Signaling-Mediated Neuroinflammation
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lisa (Verified Customer) (10-04-2021)
The antibody works quite good on hiPSCs-derived neurons. The signal is strong. The obtained bands are not very specific but the signal itself it is, therefore is easy to analyze the data.