|Positive WB detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
19953-1-AP targets NMDAR2A/GRIN2A in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||glutamate receptor, ionotropic, N-methyl D-aspartate 2A|
|Calculated molecular weight||165 kDa|
|Observed molecular weight||150-160 kDa|
|GenBank accession number||NM_000833|
|Gene ID (NCBI)||2903|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRIN2A, also named as NMDAR2A and NR2A, belongs to the glutamate-gated ion channel (TC 1.A.10) family. GRIN2A is NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation of GRIN2A requires binding of agonist to both types of subunits. The antibody recognize the C-term of GRIN2A.
|Product Specific Protocols|
|WB protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|IHC protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|IF protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|FC protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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