NMDAR2A/GRIN2A Polyclonal antibody

NMDAR2A/GRIN2A Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 19953-1-AP

Synonyms

GluN2A, GRIN2A, hNR2A, NMDAR2A, NR2A



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19953-1-AP targets NMDAR2A/GRIN2A in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2A
Calculated molecular weight 165 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberNM_000833
Gene symbol GRIN2A
Gene ID (NCBI) 2903
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRIN2A, also named as NMDAR2A and NR2A, belongs to the glutamate-gated ion channel (TC 1.A.10) family. GRIN2A is NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation of GRIN2A requires binding of agonist to both types of subunits. The antibody recognize the C-term of GRIN2A.

Protocols

Product Specific Protocols
WB protocol for NMDAR2A/GRIN2A antibody 19953-1-APDownload protocol
IHC protocol for NMDAR2A/GRIN2A antibody 19953-1-APDownload protocol
IF protocol for NMDAR2A/GRIN2A antibody 19953-1-APDownload protocol
FC protocol for NMDAR2A/GRIN2A antibody 19953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochim Biophys Acta

Specificity Protein 4 functionally regulates the transcription of NMDA receptor subunits GluN1, GluN2A, and GluN2B.

Authors - Priya Anusha A
humanWB

EBioMedicine

Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.

Authors - Qin Yang
mouseWB

Neurochem Res

Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model.

Authors - Lan Tang
humanWB

Biochem Biophys Res Commun

The second PDZ domain of scaffold protein Frmpd2 binds to GluN2A of NMDA receptors.

Authors - Xi Lu
mouseWB

Brain Res

Central nervous system-specific knockout of Brg1 causes growth retardation and neuronal degeneration.

Authors - Li Deng
mouseWB,IF

Neurotox Res

Corticosterone Induced the Increase of proBDNF in Primary Hippocampal Neurons Via Endoplasmic Reticulum Stress.

Authors - Yu Liu