|Positive WB detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
19953-1-AP targets NMDAR2A/GRIN2A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||glutamate receptor, ionotropic, N-methyl D-aspartate 2A|
|Calculated molecular weight||165 kDa|
|Observed molecular weight||150-160 kDa|
|GenBank accession number||NM_000833|
|Gene ID (NCBI)||2903|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRIN2A, also named as NMDAR2A and NR2A, belongs to the glutamate-gated ion channel (TC 1.A.10) family. GRIN2A is NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation of GRIN2A requires binding of agonist to both types of subunits. The antibody recognize the C-term of GRIN2A.
|Product Specific Protocols|
|WB protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|IHC protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|IF protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|FC protocol for NMDAR2A/GRIN2A antibody 19953-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
Specificity Protein 4 functionally regulates the transcription of NMDA receptor subunits GluN1, GluN2A, and GluN2B.
Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.
Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice.
Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model.
Biochem Biophys Res Commun
The second PDZ domain of scaffold protein Frmpd2 binds to GluN2A of NMDA receptors.
Central nervous system-specific knockout of Brg1 causes growth retardation and neuronal degeneration.