|Positive WB detected in||mouse brain tissue, human brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21920-1-AP is NMDAR2B/GRIN2B Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NMDAR2B/GRIN2B fusion protein Ag16318|
|Full Name||glutamate receptor, ionotropic, N-methyl D-aspartate 2B|
|Calculated molecular weight||1484 aa, 166 kDa|
|Observed molecular weight||166 kDa|
|GenBank accession number||BC113620|
|Gene ID (NCBI)||2904|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.
|Product Specific Protocols|
|WB protocol for NMDAR2B/GRIN2B antibody 21920-1-AP||Download protocol|
|IHC protocol for NMDAR2B/GRIN2B antibody 21920-1-AP||Download protocol|
|IF protocol for NMDAR2B/GRIN2B antibody 21920-1-AP||Download protocol|
|IP protocol for NMDAR2B/GRIN2B antibody 21920-1-AP||Download protocol|
|FC protocol for NMDAR2B/GRIN2B antibody 21920-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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