NMDAR2B/GRIN2B Polyclonal antibody

NMDAR2B/GRIN2B Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21920-1-AP

Synonyms

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21920-1-AP is NMDAR2B/GRIN2B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebra finch
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMDAR2B/GRIN2B fusion protein Ag16318
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2B
Calculated molecular weight 1484 aa, 166 kDa
Observed molecular weight 166 kDa
GenBank accession numberBC113620
Gene symbol GRIN2B
Gene ID (NCBI) 2904
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Protocols

Product Specific Protocols
WB protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
IHC protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
IF protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
IP protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
FC protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurosci Lett

Inhibition of CB1 receptor ameliorates spatial learning and memory impairment in mice with traumatic brain injury.

Authors - Xiaoming Xu
ratWB

Biochem Biophys Res Commun

Inhibitory effects of glucagon-like peptide-1 receptor on epilepsy.

Authors - Yuetao Wen
mouseWB

J Neurosci

CAMK2-dependent signaling in neurons is essential for survival.

Authors - Martijn J Kool
ratIHC

Clin Sci (Lond)

Prenatal cold exposure causes hypertension in offspring by hyperactivity of the sympathetic nervous system.

Authors - Ken Chen
ratWB

Brain Res

PMID: 31121159

Authors - Xu Wang
zebra finchWB

Behav Brain Res

Social context-dependent singing alters molecular markers of dopaminergic and glutamatergic signaling in finch basal ganglia Area X.

Authors - Lisa Y So