NMDAR2B/GRIN2B Polyclonal antibody

• Featured Product
• KD/KO Validated

NMDAR2B/GRIN2B Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

101

Cat no : 21920-1-AP

Synonyms

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

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Validation Data Gallery

• 

  WB analysis using 21920-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21920-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21920-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21920-1-AP

  IP result of anti-NMDAR2B/GRIN2B (IP:21920-1-AP, 3ug; Detection:21920-1-AP 1:2000) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of mouse brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 21920-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2B/GRIN2B antibody (21920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 21920-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug NMDAR2B/GRIN2B antibody (21920-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 93 publications below
• IHC: See 8 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

21920-1-AP targets NMDAR2B/GRIN2B in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebra finch
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMDAR2B/GRIN2B fusion protein Ag16318
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Calculated Molecular Weight: 1484 aa, 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: BC113620
• Gene Symbol: GRIN2B
• Gene ID (NCBI): 2904
• RRID: AB_11232223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Single-Nucleus RNA Sequencing Reveals that Decorin Expression in the Amygdala Regulates Perineuronal Nets Expression and Fear Conditioning Response after Traumatic Brain Injury.; Authors - Yingwu Shi
mouse	WB	Sci Adv; Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission; Authors - Wenquan Liang
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
human	WB,CoIP	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
mouse	WB,IF,IP	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach

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