NMDAR2B/GRIN2B Monoclonal antibody

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• NMDAR2B/GRIN2B Monoclonal antibody (66565-1-Ig)

NMDAR2B/GRIN2B Monoclonal Antibody for WB, ELISA

Cat No. 66565-1-Ig Copied!

Clone No. 1C5E12

Publications (4)

Reviews (1)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, ELISA

Synonyms

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

• 

  WB analysis of mouse brain using 66565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 66565-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66565-1-Ig targets NMDAR2B/GRIN2B in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16718

  Product name: Recombinant human GRIN2B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1133-1485 aa of BC113620

  Sequence: YLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHIKHGTGDKHGVVSGVPAPWEKNLTNVEWEDRSGGNFCRSCPSKLHNYSTTVTGQNSGRQACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAVTSNASTTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFMDGSPYAHMFEMSAGESTFANNKSSVPTAGHHHHNNPGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGASKARPDFRALVTNKPVVSALHGAVPARFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV

• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Calculated Molecular Weight: 1484 aa, 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: BC113620
• Gene Symbol: GRIN2B
• Gene ID (NCBI): 2904
• RRID: AB_2881926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13224
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Publications

What published studies show

The NMDAR2B/GRIN2B Monoclonal antibody (1C5E12), catalog number 66565-1-Ig, has 4 total citations. These appear in Nature, Neuron, Molecular Cell, and Philosophical Transactions of the Royal Society B. The research fields span autism spectrum disorder, NMDA receptor channel gating, circRNA-mediated translational repression, and synaptic plasticity in fragile X syndrome. All citations used the antibody for Western blot in human, mouse, or rat samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; An X-linked long non-coding RNA, PTCHD1-AS, and the core features of autism; Authors - Clarrisa A Bradley
rat	WB	Neuron; Structural basis for channel gating and blockade in tri-heteromeric GluN1-2B-2D NMDA receptor; Authors - Hyunook Kang
human	WB	Mol Cell; Global mapping of circRNA-target RNA interactions reveal P-body-mediated translational repression.; Authors - Peng Li
mouse	WB	Philos Trans R Soc Lond B Biol Sci; Cage effects on synaptic plasticity and its modulation in a mouse model of fragile X syndrome; Authors - Rasa Volianskis

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

One review rated this antibody 4 out of 5 stars for Western Blot on hiPSCs-derived neurons at a 1:1000 dilution. The reviewer noted that the signal is not very strong or specific in this cell type—it is detectable with approximately 90 seconds of exposure, but occasionally produces aspecific bands. Overall, the antibody works but may require optimization for sensitive or low-abundance targets in neuronal applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Lisa (Verified Customer) (10-04-2021)

The antibody signal is not very strong and specific on hiPSCs derived neurons: it is detectable with an exposure of about 90 seconds, but sometimes gives aspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons