GRIPAP1 Polyclonal antibody

GRIPAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15806-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA1167, GRIP1-associated protein 1, GRIP1 associated protein 1, GRASP-1 C-terminal chain, GRASP-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHCIF/ICC

WB analysis using 15806-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15806-1-AP (GRIPAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 15806-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15806-1-AP (GRIPAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 15806-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15806-1-AP (GRIPAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of SH-SY5Y using 15806-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GRIPAP1 antibody (15806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for GRIPAP1 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15806-1-AP targets GRIPAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8540 Product name: Recombinant human GRIPAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 292-587 aa of BC001522 Sequence: QEERDCHLKTISSLKQEVKDTVDGQRILEKKGSAALKDLKRQLHLERKRADKLQERLQDILTNSKSRSGLEELVLSEMNSPSRTQTGDSSSISSFSYREILREKESSAVPARSLSSSPQAQPPRPAELSDEEVAELFQRLAETQQEKWMLEEKVKHLEVSSASMAEDLCRKSAIIETYVMDSRIDVSVAAGHTDRSGLGSVLRDLVKPGDENLREMNKKLQNMLEEQLTKNMHLHKDMEVLSQEIVRLSKECVGPPDPDLEPGETS Predict reactive species
Full Name	GRIP1 associated protein 1
Calculated Molecular Weight	841 aa, 96 kDa
Observed Molecular Weight	97 kDa
GenBank Accession Number	BC001522
Gene Symbol	GRIPAP1
Gene ID (NCBI)	56850
RRID	AB_2112787
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q4V328
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIP-associated protein-1 (GRASP-1), also named as GRIPAP1, is a neuron-specific effector of the small GTPase Rab4 and key component of AMPA receptor recycling machinery in dendrites. GRASP-1 is essential for maintenance of spine morphology and important for LTP. GRASP-1 connects Rab4 and Rab11 recycling endosomal domains through the interaction with target (t)-SNARE syntaxin. The MW of this protein is 97 kDa, and Catalog#15806-1-AP specially recognises the 97 kDa protein.

Protocols

Product Specific Protocols
IF protocol for GRIPAP1 antibody 15806-1-AP	Download protocol
IHC protocol for GRIPAP1 antibody 15806-1-AP	Download protocol
WB protocol for GRIPAP1 antibody 15806-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The GRIPAP1 Polyclonal antibody (Cat# 15806-1-AP) has 1 total citation. It appears in Cell (IF 45.1), in a study on modulated expression of specific tRNAs driving gene expression and cancer progression. The cited application was Western Blot in human samples, placing it within the fields of molecular biology, neuroscience, and oncology.