Tested Applications
| Positive WB detected in | Y79 cells, mouse eye tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24606-1-AP targets GRK1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18063 Product name: Recombinant human GRK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 339-563 aa of BC140945 Sequence: AVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS Predict reactive species |
| Full Name | G protein-coupled receptor kinase 1 |
| Calculated Molecular Weight | 563 aa, 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC140945 |
| Gene Symbol | GRK1 |
| Gene ID (NCBI) | 6011 |
| RRID | AB_2879635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15835 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GRK1 antibody 24606-1-AP | Download protocol |
| IHC protocol for GRK1 antibody 24606-1-AP | Download protocol |
| IP protocol for GRK1 antibody 24606-1-AP | Download protocol |
| WB protocol for GRK1 antibody 24606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GRK1 Polyclonal antibody (Cat# 24606-1-AP) has 6 citations published in Scientific Advances, Zoological Research (×2), Biochimica et Biophysica Acta – Molecular Basis of Disease, FEBS Journal, and Vision Research. The cited studies focus on retinal degeneration, photoreceptor biology, ciliary architecture, ciliopathy models, and protein trafficking in mouse and zebrafish systems, with applications including WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Sci Adv Synergistic antioxidant and gene supplementation for high-efficacy retinitis pigmentosa therapy. | ||
Zool Res Tubulin hyperglutamylation induces retinal degeneration by disrupting ciliary architecture and protein trafficking. | ||
Zool Res Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic Sdccag8 mutations. | ||
Biochim Biophys Acta Mol Basis Dis Knockout of DLIC1 leads to retinal cone degeneration via disturbing Rab8 transport in zebrafish | ||
