GRP78,BIP Recombinant monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 80849-1-RR (same clone as 80849-1-PBS)

  WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  WB analysis using 80849-1-RR (same clone as 80849-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80849-1-RR (same clone as 80849-1-PBS)

  IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80849-1-RR (same clone as 80849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80849-1-RR (same clone as 80849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 80849-1-RR (same clone as 80849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80849-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.

• Featured Product
• KD/KO Validated

GRP78,BIP Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 80849-1-PBS

Clone No.5F17

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

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Product Information

80849-1-PBS targets GRP78,BIP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2188

  Product name: Recombinant human GRP78,BIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 351-654 aa of BC020235

  Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL

• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70,78 kDa
• GenBank Accession Number: BC020235
• Gene Symbol: GRP78/BIP
• Gene ID (NCBI): 3309
• RRID: AB_2918916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11021
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.