GRP94 Recombinant monoclonal antibody

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  WB analysis using 82805-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82805-1-RR (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82805-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82805-1-RR against Human GRP94 were performed. The affinity constant is 1.30 nM.

GRP94 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82805-1-RR

Clone No.6K5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82805-1-RR targets GRP94 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6344

  Product name: Recombinant human GRP94 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 455-803 aa of BC066656

  Sequence: KLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTAEKDEL

• Full Name: heat shock protein 90kDa beta (Grp94), member 1
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 94-100 kDa
• GenBank Accession Number: BC066656
• Gene Symbol: GRP94
• Gene ID (NCBI): 7184
• RRID: AB_3086543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14625
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.