GRWD1 Polyclonal antibody

GRWD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 10354-1-AP

Synonyms

GRWD, GRWD1, KIAA1942, RRB1, WDR28

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10354-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 10354-1-AP

  IP result of anti-GRWD1 (IP:10354-1-AP, 4ug; Detection:10354-1-AP 1:1000) with PC-3 cells lysate 4000ug.

• 

  IHC staining of human prostate cancer using 10354-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10354-1-AP (GRWD1 antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, SKOV-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

10354-1-AP targets GRWD1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRWD1 fusion protein Ag0367
• Full Name: glutamate-rich WD repeat containing 1
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC002440
• Gene Symbol: GRWD1
• Gene ID (NCBI): 83743
• RRID: AB_2114642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. [PMID:15885502]

Publications

Species	Application	Title
human	WB,IHC	Exp Cell Res; GRWD1 promotes cell proliferation and migration in non-small cell lung cancer by activating the Notch pathway.; Authors - Qiongzi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zehua (Verified Customer) (12-17-2019)

I identified siRNAs targeting GRWD1 with this antibody. It works well for WB. I strongly suggested it.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7

GRWD1 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in MCF-7 (Cat no:10354-1-AP)