Tested Applications
| Positive WB detected in | HEK-293 cells, PC-3 cells, SKOV-3 cells |
| Positive IP detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10354-1-AP targets GRWD1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0367 Product name: Recombinant human GRWD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-236 aa of BC002440 Sequence: AESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSEGSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTG Predict reactive species |
| Full Name | glutamate-rich WD repeat containing 1 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC002440 |
| Gene Symbol | GRWD1 |
| Gene ID (NCBI) | 83743 |
| RRID | AB_2114642 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQ67 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. (PMID:15885502)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GRWD1 antibody 10354-1-AP | Download protocol |
| WB protocol for GRWD1 antibody 10354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GRWD1 Polyclonal antibody (catalog number 10354-1-AP) has 2 total citations. These appear in Microbiology Spectrum and Experimental Cell Research. The associated research fields include virology (HSV-1 replication and nuclear egress) and cancer biology (non-small cell lung cancer, cell proliferation, migration, and Notch pathway activation). Both citations involve human samples using WB, CoIP, and IHC applications.
| Species | Application | Title |
|---|---|---|
Microbiol Spectr GRWD1 enhances HSV-1 replication by facilitating nuclear egress.
| ||
Exp Cell Res GRWD1 promotes cell proliferation and migration in non-small cell lung cancer by activating the Notch pathway. |
Reviews
What customers say
The single review for 10354-1-AP is a 5-star rating. The user applied the antibody for Western Blot on MCF-7 cell culture lysate at a 1:1000 dilution and reported it performed well. They used it to identify siRNAs targeting GRWD1 and strongly recommended the product. Overall, the feedback is positive, highlighting reliable WB performance and successful use in their target-validation workflow.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zehua (Verified Customer) (12-17-2019) | I identified siRNAs targeting GRWD1 with this antibody. It works well for WB. I strongly suggested it.
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