GSDMB Polyclonal antibody

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  WB analysis of A431 using 12885-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

GSDMB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12885-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

Gasdermin-like protein, Gasdermin-B, N-terminal, Gasdermin-B, C-terminal, Gasdermin-B, Gasdermin like protein

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

12885-1-AP targets GSDMB in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSDMB fusion protein Ag3895
• Full Name: gasdermin B
• Calculated Molecular Weight: 411 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC025682
• Gene Symbol: GSDMB
• Gene ID (NCBI): 55876
• RRID: AB_2114762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TAX9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
Human		Am J Respir Cell Mol Biol; Glutaminolysis Epigenetically Regulates Anti-Apoptotic Gene Expression in IPF Fibroblasts.; Authors - Le Bai
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu ; KD Validated
human	WB, IHC	Int J Biol Sci; USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway.; Authors - Haiqing He
human	IHC	Front Oncol; Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma.; Authors - Jia You
		J Biol Chem; Glutaminolysis is required for transforming growth factor-β1-induced myofibroblast differentiation and activation.; Authors - Karen Bernard
human	WB	J Allergy Clin Immunol; A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis.; Authors - Ronald A Panganiban