GSDMB Polyclonal antibody

GSDMB Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12885-1-AP

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

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Validation Data Gallery

• 

  WB analysis of COLO 320 using 12885-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12885-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

12885-1-AP targets GSDMB in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSDMB fusion protein Ag3895
• Full Name: gasdermin B
• Calculated Molecular Weight: 411 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC025682
• Gene Symbol: GSDMB
• Gene ID (NCBI): 55876
• RRID: AB_2114762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu ; KD Validated
human	WB, IHC	Int J Biol Sci; USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway.; Authors - Haiqing He
human	IHC	Front Oncol; Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma.; Authors - Jia You
human	WB	Toxicol Appl Pharmacol; Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity; Authors - Anping Song
human	IHC,IF	Cell Death Dis; Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence; Authors - Ying Zhang
human	WB	J Allergy Clin Immunol; A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis.; Authors - Ronald A Panganiban