GSG1L Polyclonal antibody

GSG1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17328-1-AP

Synonyms

GSG1 like, GSG1 like protein, GSG1L, PRO19651

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Validation Data Gallery

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  WB analysis of U-251 using 17328-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 17328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 17328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Raji cells, U-251 cells, SGC-7901 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17328-1-AP targets GSG1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSG1L fusion protein Ag11307
• Full Name: GSG1-like
• Calculated Molecular Weight: 176 aa, 21 kDa
• Observed Molecular Weight: 36-43 kDa
• GenBank Accession Number: BC016460
• Gene Symbol: GSG1L
• Gene ID (NCBI): 146395
• RRID: AB_2878384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSG1L is a component of the inner core of AMPAR complex. It modifies AMPA receptor (AMPAR) gating. GSG1L is mainly present on B lymphocytes. This antibody can recognize all the 4 isoforms of GSG1L. GSG1L was detected the isoform of 43 kDa in rat brain (PMID: 22813734).

Publications

Species	Application	Title
rat		Nat Commun; GSG1L-containing AMPA receptor complexes are defined by their spatiotemporal expression, native interactome and allosteric sites; Authors - Amanda M Perozzo