|Positive WB detected in||HeLa cells, HEK-293 cells, A549 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
51065-1-AP targets GSK3B in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||glycogen synthase kinase 3 beta|
|Calculated molecular weight||433 aa, 48 kDa|
|Observed molecular weight||46-52 kDa|
|GenBank accession number||BC000251|
|Gene ID (NCBI)||2932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.
|Product Specific Protocols|
|WB protocol for GSK3B antibody 51065-1-AP||Download protocol|
|IHC protocol for GSK3B antibody 51065-1-AP||Download protocol|
|IF protocol for GSK3B antibody 51065-1-AP||Download protocol|
|IP protocol for GSK3B antibody 51065-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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