GSK3B Polyclonal antibody

• Featured Product
• KD/KO Validated

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 51065-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

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Validation Data Gallery

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  WB analysis of A549 using 51065-1-AP

  WB result of GSK3B antibody (51065-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

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  WB analysis of HeLa using 51065-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51065-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51065-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51065-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 51065-1-AP

  IP result of anti-GSK3B (IP:51065-1-AP, 3ug; Detection:51065-1-AP 1:500) with HeLa cells lysate 920ug.

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  IHC staining of human prostate cancer using 51065-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51065-1-AP (GSK3B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 51065-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51065-1-AP (GSK3B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of NIH/3T3 using 51065-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 51065-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 51065-1-AP

  Immunofluorescent analysis of HeLa cells using 51065-1-AP (GSK3B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, A549 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below

Product Information

51065-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 46-52 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• RRID: AB_2232770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.

Publications

Species	Application	Title
human	WB	Cell Death Differ; RPS23RG1 modulates tau phosphorylation and axon outgrowth through regulating p35 proteasomal degradation.; Authors - Dongdong Zhao
mouse	WB	J Steroid Biochem Mol Biol; High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.; Authors - Rui Wang
mouse	IF	Mol Biol Cell; ILK modulates epithelial polarity and matrix formation in hair follicles.; Authors - Alena Rudkouskaya
human	WB	Biochem Biophys Res Commun; N-glycosylation of CD82 at Asn157 is required for suppressing migration and invasion by reversing EMT via Wnt/β-catenin pathway in colon cancer; Authors - Lin Zhu
human	WB	Front Pharmacol; Chebulagic acid suppresses gastric cancer by inhibiting the AURKA/β-catenin/Wnt pathway; Authors - Jing Zhao
human	WB	Int J Dev Neurosci; AQP8 may affect glioma proliferation and growth by regulating GSK-3β phosphorylation and nuclear transport of β-catenin; Authors - Ziling Cai