Multi-rAb® GSK3B Multi-Recombinant antibody

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• Multi-rAb® GSK3B Multi-Recombinant antibody (RMX00019)

GSK3B Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. RMX00019

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GSK3 beta, EC:2.7.11.1, EC:2.7.11.26, GSK 3 beta, GSK-3 beta

• 

  WB analysis using RMX00019

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00019 (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using RMX00019

  IP result of anti-GSK3B (IP:RMX00019, 4ug; Detection:RMX00019 1:20000) with HeLa cells lysate 960 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skin using RMX00019

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using RMX00019 (GSK3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using RMX00019

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (RMX00019) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, PC-12 cells, NIH/3T3 cells, PC-3 cells, mouse thymus tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00019 targets GSK3B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Mixture

• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 46-48 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• RRID: AB_3750078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P49841
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.