Tested Applications
| Positive WB detected in | A549 cells, U2OS cells, HeLa cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11644-2-AP targets Gelsolin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2239 Product name: Recombinant human Gelsolin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 433-782 aa of BC026033 Sequence: MAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA Predict reactive species |
| Full Name | gelsolin (amyloidosis, Finnish type) |
| Calculated Molecular Weight | 782 aa, 86 kDa |
| Observed Molecular Weight | 87-93 kDa |
| GenBank Accession Number | BC026033 |
| Gene Symbol | Gelsolin |
| Gene ID (NCBI) | 2934 |
| RRID | AB_2295090 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06396 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Plasma gelsolin can interact with actin and form a complex of 130 kDa (PMID: 6330060, 3030756).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Gelsolin antibody 11644-2-AP | Download protocol |
| WB protocol for Gelsolin antibody 11644-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Gelsolin antibody (Cat# 11644-2-AP) has 15 citations across journals including Cell, Molecular Cell, Journal of Clinical Investigation, Science Advances, Advanced Healthcare Materials, Cell Reports, Genes & Development, Journal of Ethnopharmacology, International Journal of Molecular Sciences, Cancers, Journal of Neuroscience, American Journal of Cancer Research, Gene, Biomedical Pharmacotherapy, and Oncotarget. Research fields span Alzheimer's disease, ciliary biology, cardiac sepsis, bone regeneration, cochlear development, heart failure, and multiple cancers (bladder, ovarian, lung, hepatocellular, nasopharyngeal).
| Species | Application | Title |
|---|---|---|
Cell Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. | ||
Mol Cell Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. | ||
J Clin Invest Loss of RPGR disrupts motile cilia and causes primary ciliary dyskinesia by affecting F-actin dynamics. | ||
Sci Adv Cytoskeletal remodeling promotes tunneling nanotube formation and drives cardiac resident cell mitochondrial transfer in sepsis. | ||
Adv Healthc Mater Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein. | ||
Cell Rep Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. |
Reviews
What customers say
The single review for this product is a 5-star rating from an immunohistochemistry application. The reviewer used a 1:1000 dilution on human post-mortem FFPE brain tissue and reported that the antibody is easy to use and clearly reveals the Gelsolin antigen. Overall, the reviewer expressed full satisfaction with the reference.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jean-Loup (Verified Customer) (01-22-2026) | Good antibody, easy to use, reveals well the antigen Gelsolin protein in human post-mortem FFPE brain. I am fully satisfied by this reference
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