GSR Polyclonal antibody

• Featured Product
• KD/KO Validated

GSR Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 18257-1-AP

Synonyms

GLUR, glutathione reductase, GRase, GRD1, GSR

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Validation Data Gallery

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  WB analysis of HeLa using 18257-1-AP

  WB result of GSR antibody (18257-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSR transfected HeLa cells.

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  WB analysis using 18257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18257-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18257-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18257-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18257-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18257-1-AP

  IP Result of anti-GSR (IP:18257-1-AP, 4ug; Detection:18257-1-AP 1:500) with HeLa cells lysate 2600ug.

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  IHC staining of human breast cancer using 18257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 18257-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GSR antibody 18257-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, mouse lung tissue, human placenta tissue, mouse testis tissue, rat testis tissue
• Positive IP detected in: HeLa cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below

Product Information

18257-1-AP targets GSR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSR fusion protein Ag13080
• Full Name: glutathione reductase
• Calculated molecular weight: 522 aa, 56 kDa
• Observed molecular weight: 52-55 kDa
• GenBank accession number: BC069244
• Gene symbol: GSR
• Gene ID (NCBI): 2936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.

Publications

Species	Application	Title
human	WB	Redox Biol; Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.; Authors - Angel Gallego-Selles
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
human	WB	Nutrients; A Mango Leaf Extract (Zynamite®) Combined with Quercetin Has Exercise-Mimetic Properties in Human Skeletal Muscle; Authors - Miriam Martinez-Canton
human	WB	Pharmacol Res; Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.; Authors - Meng-Ting Chang
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong
mouse	WB	Free Radic Biol Med; Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.; Authors - Jan Blecha