• Featured Product
  • KD/KO Validated

GSR Polyclonal antibody

GSR Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 18257-1-AP

Synonyms

GLUR, glutathione reductase, GRase, GRD1, GSR



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse lung tissue, human placenta tissue, mouse testis tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18257-1-AP targets GSR in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSR fusion protein Ag13080
Full Name glutathione reductase
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC069244
Gene symbol GSR
Gene ID (NCBI) 2936
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.

Protocols

Product Specific Protocols
WB protocol for GSR antibody 18257-1-APDownload protocol
IF protocol for GSR antibody 18257-1-APDownload protocol
IP protocol for GSR antibody 18257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Sci Food Agric

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Authors - Zhengxuan Wang
humanWB

Cell Cycle

GSH-dependent antioxidant defense contributes to the acclimation of colon cancer cells to acidic microenvironment.

Authors - Minnan Zhao
  • KD Validated
ratWB

J Food Biochem

Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.

Authors - Zhengxuan Wang
ratWB

Drug Des Devel Ther

PMID: 32308368

Authors - Yan Zheng
humanWB

Sci Rep

PMID: 27193186

Authors - Zhi-Guo Zheng
mouseWB

Free Radic Biol Med

Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.

Authors - Jan Blecha