Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, mouse lung tissue, human placenta tissue, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18257-1-AP targets GSR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13080 Product name: Recombinant human GSR protein Source: e coli.-derived, PKG Tag: GST Domain: 274-522 aa of BC069244 Sequence: ISTNCTEELENAGVEVLKFSQVKEVKKTLSGLEVSMVTAVPGRLPVMTMIPDVDCLLWAIGRVPNTKDLSLNKLGIQTDDKGHIIVDEFQNTNVKGIYAVGDVCGKALLTPVAIAAGRKLAHRLFEYKEDSKLDYNNIPTVVFSHPPIGTVGLTEDEAIHKYGIENVKTYSTSFTPMYHAVTKRKTKCVMKMVCANKEEKVVGIHMQGLGCDEMLQGFAVAVKMGATKADFDNTVAIHPTSSEELVTLR Predict reactive species |
| Full Name | glutathione reductase |
| Calculated Molecular Weight | 522 aa, 56 kDa |
| Observed Molecular Weight | 52-55 kDa |
| GenBank Accession Number | BC069244 |
| Gene Symbol | GSR |
| Gene ID (NCBI) | 2936 |
| RRID | AB_10598162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00390 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSR antibody 18257-1-AP | Download protocol |
| IHC protocol for GSR antibody 18257-1-AP | Download protocol |
| IP protocol for GSR antibody 18257-1-AP | Download protocol |
| WB protocol for GSR antibody 18257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSR Polyclonal antibody (Cat# 18257-1-AP) has 41 citations. These appear in journals including Cell, Nature Communications, Redox Biology, Molecular Cell, Free Radical Biology and Medicine, Cell Reports, EBioMedicine, Nature Metabolism, and Journal of Biological Chemistry. Research fields span redox biology, oxidative stress, glutathione metabolism, ferroptosis, antioxidant defense, cancer, osteoclastogenesis/bone loss, neurodegeneration, and diabetes.
| Species | Application | Title |
|---|---|---|
Nat Commun A family of NADPH/NADP+ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes | ||
Redox Biol Exacerbated response to oxidative stress in the Retinitis Pigmentosa CerklKD/KO mouse model triggers retinal degeneration pathways upon acute light stress | ||
Redox Biol Glutathione reductase underlies the stability of mutant p53 by antagonizing protein glutathionylation | ||
Redox Biol Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation. | ||
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used it for Western Blot on mouse liver tissue at a 1:2000 dilution and described it as a good antibody that is very specific to GSR with a very clean background. Overall, the feedback highlights strong specificity and low background, indicating reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (08-15-2024) | Good antibody. Very specific to GSR. Very clean background.
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