Validation Data Gallery
|Positive WB detected in||Jurkat cells, HeLa cells, mouse lung tissue, human placenta tissue, mouse testis tissue, rat testis tissue|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
18257-1-AP targets GSR in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GSR fusion protein Ag13080|
|Full Name||glutathione reductase|
|Calculated molecular weight||522 aa, 56 kDa|
|Observed molecular weight||52-55 kDa|
|GenBank accession number||BC069244|
|Gene ID (NCBI)||2936|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.
J Sci Food Agric
l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.
GSH-dependent antioxidant defense contributes to the acclimation of colon cancer cells to acidic microenvironment.
J Food Biochem
Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.
Drug Des Devel Ther
Free Radic Biol Med
Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.