GSR Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84480-2-RR (same clone as 84480-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84480-2-RR (GSR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84480-2-RR (same clone as 84480-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSR antibody (84480-2-RR, Clone: 241934D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84480-2-RR (same clone as 84480-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GSR Recombinant antibody (84480-2-RR, Clone:241934D2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84480-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84480-2-RR against Human GSR were performed. The affinity constant is below 1 pM.

GSR Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84480-2-PBS

Clone No.241934D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

241934D2, EC:1.8.1.7, GLUR, glutathione reductase, GRase

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Product Information

84480-2-PBS targets GSR in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13080

  Product name: Recombinant human GSR protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 274-522 aa of BC069244

  Sequence: ISTNCTEELENAGVEVLKFSQVKEVKKTLSGLEVSMVTAVPGRLPVMTMIPDVDCLLWAIGRVPNTKDLSLNKLGIQTDDKGHIIVDEFQNTNVKGIYAVGDVCGKALLTPVAIAAGRKLAHRLFEYKEDSKLDYNNIPTVVFSHPPIGTVGLTEDEAIHKYGIENVKTYSTSFTPMYHAVTKRKTKCVMKMVCANKEEKVVGIHMQGLGCDEMLQGFAVAVKMGATKADFDNTVAIHPTSSEELVTLR

• Full Name: glutathione reductase
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC069244
• Gene Symbol: GSR
• Gene ID (NCBI): 2936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00390
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.