GST Tag Recombinant antibody

GST Tag Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Recombinant protein

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

9B24

Cat no : 80006-1-RR

Synonyms

Glutathione S-Transferase, GST, GST Tag

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Validation Data Gallery

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  WB analysis using 80006-1-RR

  GST tagged protein and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• CoIP: See 1 publications below

Product Information

80006-1-RR targets GST Tag in WB, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.

• Tested Reactivity: Recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GST Tag fusion protein Ag0040
• Full Name: GST Tag
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: FN315687
• RRID: AB_2882936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.

Publications

Species	Application	Title
	CoIP	Vet Microbiol; PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication; Authors - Huanjie Zhai