ChromoTek GST Monoclonal antibody (6G9)
anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins
Specificity
Spot-Tag
Applications
IF, WB
Conjugate
0.5 – 1 mg/mL (conjugates)
Cat no : 6g9
Synonyms
Validation Data Gallery
Product Information
anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins
| Applications | IF, WB |
| Recommended Dilution | IF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 6 nM |
| Specificity/Target | Spot-Tag (PDRVRAVSHWSS) |
| GeneID (NCBI) | 0 |
| Conjugate | 0.5 – 1 mg/mL (conjugates) |
| Storage Buffer | Buffered aqueous solution (PBS) |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C / 40°F.Stable for 6 months. Do not freeze. Protect from light. |
Documentation
| SDS |
|---|
| SDS Spot-Label ATTO488 |
| Protocol |
|---|
| Protocol Spot-Label ATTO488 |
| Brochure |
|---|
| Product brochure (PDF) |
Publications
| Application | Title |
|---|---|
Nat Commun ATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis. |