Tested Applications
| Positive WB detected in | human brain tissue, HEK-293T cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17271-1-AP targets GSTA4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11028 Product name: Recombinant human GSTA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC015523 Sequence: MAARPKLHYPNGRGRMESVRWVLAAAGVEFDEEFLETKEQLYKLQDGNHLLFQQVPMVEIDGMKLVQTRSILHYIADKHNLFGKNLKERTLIDMYVEGTLDLLELLIMHPFLKPDDQQKEVVNMAQKAIIRYFPVFEKILRGHGQSFLVGNQLSLADVILLQTILALEEKIPNILSAFPFLQEYTVKLSNIPTIKRFLEPGSKKKPPPDEIYVRTVYNIFRP Predict reactive species |
| Full Name | glutathione S-transferase alpha 4 |
| Calculated Molecular Weight | 222 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC015523 |
| Gene Symbol | GSTA4 |
| Gene ID (NCBI) | 2941 |
| RRID | AB_2295133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15217 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSTA4 (Glutathione S-Transferase Alpha 4) is an enzyme that plays a key role in detoxification and antioxidant defense. Its structure includes a GSH-binding site and a hydrophobic substrate-binding domain, functioning as a dimer. Dysregulation of GSTA4 is linked to diseases like cancer, neurodegeneration, and diabetes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTA4 antibody 17271-1-AP | Download protocol |
| IP protocol for GSTA4 antibody 17271-1-AP | Download protocol |
| WB protocol for GSTA4 antibody 17271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTA4 Polyclonal antibody (Cat# 17271-1-AP) has 13 citations published in journals including Cell Metabolism, Free Radical Biology and Medicine, Mechanisms of Ageing and Development, Journal of Molecular Medicine, Journal of Lipid Research, Experimental and Molecular Pathology, PLoS One, Bioscience Microbiota Food and Health, Journal of Toxicologic Pathology, bioRxiv, Ecotoxicology and Environmental Safety, and Advanced Science. Research fields span lipid metabolism, oxidative stress, liver disease, neuroprotection, ferroptosis, prostate cancer, and placental dysfunction.
| Species | Application | Title |
|---|---|---|
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Free Radic Biol Med Increased hepatocellular protein carbonylation in human end-stage alcoholic cirrhosis. | ||
Mech Ageing Dev WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus | ||
J Mol Med (Berl) Nuclear receptors regulate lipid metabolism and oxidative stress markers in chondrocytes. | ||
J Lipid Res Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver. | ||
Exp Mol Pathol Dysregulation of antioxidant responses in patients diagnosed with concomitant Primary Sclerosing Cholangitis/Inflammatory Bowel Disease. |
Reviews
What customers say
The single review for this antibody is positive, with a 4-star rating. The user tested it by Western Blot on fallopian tube epithelial cells at a 1:500 dilution and reported that the antibody works well. Overall, the feedback indicates reliable performance for Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Iru (Verified Customer) (10-04-2021) | The antibody works well
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