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  • KD/KO Validated

GSTM1 Polyclonal antibody

GSTM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12412-1-AP

Synonyms

glutathione S transferase mu 1, GST class mu 1, GST HB subunit 4, GST1, GSTM1, GSTM1 1, GSTM1a 1a, GSTM1b 1b, GTH4, GTM1, H B, MU, MU 1



Tested Applications

Positive WB detected inmouse liver tissue, mouse ovary tissue, HeLa cells, mouse kidney tissue, rat liver
Positive IHC detected inmouse liver tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12412-1-AP targets GSTM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTM1 fusion protein Ag3113
Full Name glutathione S-transferase mu 1
Calculated Molecular Weight 181 aa, 21 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC024005
Gene Symbol GSTM1
Gene ID (NCBI) 2944
RRIDAB_2115925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSTM1 antibody 12412-1-APDownload protocol
IHC protocol for GSTM1 antibody 12412-1-APDownload protocol
IF protocol for GSTM1 antibody 12412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Immunity

Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.

Authors - Yujia Wang
  • KO Validated
humanWB

Mol Cell

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation

Authors - Wenxin Zhang
humanIHC

Acta Neuropathol

Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy.

Authors - Woutje M Berdowski
mouseWB

Sci Adv

lncRNA-GM targets Foxo1 to promote T cell-mediated autoimmunity

Authors - Yali Chen
humanWB

Autophagy

ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.

Authors - Zhiqiang Deng
humanWB

Virol Sin

Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.

Authors - Yingying Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kamal (Verified Customer) (01-26-2024)

Antibody works well at provided dilution in 1X TBST. Bands showed up around 25 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse liver tissue