mouse liver tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16766-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16766-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 16766-1-AP
IP result of anti-GSTM4 (IP:16766-1-AP, 4ug; Detection:16766-1-AP 1:500) with mouse lung tissue lysate 4000 ug.
IP result of anti-GSTM4 (IP:16766-1-AP, 4ug; Detection:16766-1-AP 1:500) with mouse lung tissue lysate 4000 ug.
IHC staining of human colon cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16766-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTM4 antibody (16766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTM4 antibody (16766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2400
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio) Hexanol Inhibits Proliferation and Induces Apoptosis of Endometriosis by Regulating Glutathione S-Transferase Mu Class 4
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16766-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16766-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse lung using 16766-1-AP
IP result of anti-GSTM4 (IP:16766-1-AP, 4ug; Detection:16766-1-AP 1:500) with mouse lung tissue lysate 4000 ug.
IF/ICC Figures
IF Staining of HepG2 using 16766-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTM4 antibody (16766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
GSTM4 Polyclonal antibody
Catalog Number
16766-1-AP
Citations
4
Dilutions
WB : 1:500-1:2400 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:1000-1:4000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
