Tested Applications
| Positive WB detected in | mouse liver tissue, HeLa cells, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16766-1-AP targets GSTM4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10340 Product name: Recombinant human GSTM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-218 aa of BC015513 Sequence: MSMTLGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPKPLYTRVAVWGNK Predict reactive species |
| Full Name | glutathione S-transferase mu 4 |
| Calculated Molecular Weight | 218 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC015513 |
| Gene Symbol | GSTM4 |
| Gene ID (NCBI) | 2948 |
| RRID | AB_2116072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03013 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTM4 antibody 16766-1-AP | Download protocol |
| IHC protocol for GSTM4 antibody 16766-1-AP | Download protocol |
| IP protocol for GSTM4 antibody 16766-1-AP | Download protocol |
| WB protocol for GSTM4 antibody 16766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTM4 Polyclonal antibody (Cat# 16766-1-AP) has 6 total citations. These appear in journals including Biomolecules, J Gerontol A Biol Sci Med Sci, Age, Discov Oncol, Reprod Sci, and Mutat Res. The associated research fields span muscle atrophy proteomics, growth hormone and aging biology, breast cancer biomarker discovery, endometriosis pathogenesis, and drug-metabolizing enzyme regulation in DNA damage models.
| Species | Application | Title |
|---|---|---|
Biomolecules Integrated Proteomic and Metabolomic Analysis of Muscle Atrophy Induced by Hindlimb Unloading. | ||
Discov Oncol Integrated analysis of single-cell and bulk RNA sequencing identifies GSTM4 as a tumor suppressor and prognostic biomarker in breast cancer. | ||
Reprod Sci 6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio) Hexanol Inhibits Proliferation and Induces Apoptosis of Endometriosis by Regulating Glutathione S-Transferase Mu Class 4 | ||
Mutat Res Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in HepG2 cells and ICR mice. |
