Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, COLO 320 cells, K-562 cells, HEK-293 cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86708-1-RR targets GSTP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag8731 Product name: Recombinant human GSTP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC010915 Sequence: MPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYVSLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ Predict reactive species |
| Full Name | glutathione S-transferase pi 1 |
| Calculated Molecular Weight | 210 aa, 23 kDa |
| Observed Molecular Weight | 23~28 kDa |
| GenBank Accession Number | BC010915 |
| Gene Symbol | GSTP1 |
| Gene ID (NCBI) | 2950 |
| RRID | AB_3745069 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09211 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutathione S-transferase Pi (GSTP1) is anisozyme encoded by the GST pi gene that plays an importantregulatory role in detoxification, anti‑oxidative damage, and the occurrence of various diseases (PMID: 14755684). GSTP1 conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PMID:9084911). GSTP1 methylation is frequently associated with tumor development or poor prognosis in a wide range of tumors such as neuroblastoma, hepatocellular carcinoma, endometrial, breast, and prostate cancers (PCa) (PMID: 27594734).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTP1 antibody 86708-1-RR | Download protocol |
| WB protocol for GSTP1 antibody 86708-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTP1 Recombinant monoclonal antibody (251705C10), catalog number 86708-1-RR, has 1 total citation. It appears in Cancers (Basel) (2026), used in Western Blot for human samples. The cited study investigates APOBEC3C's role in suppressing prostate cancer through regulation of cellular inflammation, cell cycle arrest, and DNA damage response, placing this citation within the fields of oncology and prostate cancer research.
| Species | Application | Title |
|---|---|---|
Cancers (Basel) APOBEC3C Suppresses Prostate Cancer by Regulating Key Molecules Involved in Cellular Inflammation, Cell Cycle Arrest, and DNA Damage Response. |
