GTF2E1 Polyclonal antibody

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  WB analysis of HEK-293 using 24405-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24405-1-AP (GTF2E1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 24405-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GTF2E1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24405-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FE, GTF2E1, TF2E1, TFIIE A, TFIIE alpha

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human urothelial carcinoma tissue, rat kidney tissue, mouse kidney tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

24405-1-AP targets GTF2E1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTF2E1 fusion protein Ag19794
• Full Name: general transcription factor IIE, polypeptide 1, alpha 56kDa
• Calculated Molecular Weight: 439 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC156997
• Gene Symbol: GTF2E1
• Gene ID (NCBI): 2960
• RRID: AB_2879526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29083
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	bioRxiv; Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-ends; Authors - Olivia Luyties