GTF2F2 Polyclonal antibody

GTF2F2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 15139-1-AP

Synonyms

ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta

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Validation Data Gallery

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  WB analysis of K-562 using 15139-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15139-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 15139-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15139-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15139-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 15139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15139-1-AP (GTF2F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, mouse spleen tissue, PC-3 cells, rat spleen tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15139-1-AP targets GTF2F2 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTF2F2 fusion protein Ag7313
• Full Name: general transcription factor IIF, polypeptide 2, 30kDa
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001771
• Gene Symbol: GTF2F2
• Gene ID (NCBI): 2963
• RRID: AB_2114554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.