GTF2H4 Polyclonal antibody

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  WB analysis using 15431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15431-1-AP (GTF2H4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

GTF2H4 Polyclonal Antibody for WB, ELISA

Cat No. 15431-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

TFIIH, TFB2, BTF2 p52

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Tested Applications

• Positive WB detected in: K-562 cells, mouse ovary tissue, mouse thymus tissue, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15431-1-AP targets GTF2H4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7680

  Product name: Recombinant human GTF2H4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 105-462 aa of BC004935

  Sequence: LQGLILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVSGAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS

• Full Name: general transcription factor IIH, polypeptide 4, 52kDa
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC004935
• Gene Symbol: GTF2H4
• Gene ID (NCBI): 2968
• RRID: AB_3669207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92759
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTF2H4 (General Transcription Factor IIH Subunit 4) ,also named as P52, TFB2, TFIIH, is a component of the TFIIH complex, which plays a crucial role in transcription initiation by RNA polymerase II and is also involved in nucleotide excision repair (NER). The role of GTF2H4 in DNA repair mechanisms makes it a potentially important factor in the development and progression of cancer.