GTF3A Polyclonal antibody

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• GTF3A Polyclonal antibody (33192-1-AP)

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  WB analysis using 33192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33192-1-AP (GTF3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GTF3A Polyclonal Antibody for WB, ELISA

Cat No. 33192-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TFIIIA, Transcription factor IIIA

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Tested Applications

• Positive WB detected in: LNCaP cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33192-1-AP targets GTF3A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35931

  Product name: Recombinant human GTF3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 200-365 aa of NM_002097

  Sequence: KTWTELLKHVRETHKEEILCEVCRKTFKRKDYLKQHMKTHAPERDVCRCPREGCGRTYTTVFNLQSHILSFHEESRPFVCEHAGCGKTFAMKQSLTRHAVVHDPDKKKMKLKVKKSREKRSLASHLSGYIPPKRKQGQGLSLCQNGESPNCVEDKMLSTVAVLTLG

• Full Name: general transcription factor IIIA
• Calculated Molecular Weight: 41kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_002097
• Gene Symbol: GTF3A
• Gene ID (NCBI): 2971
• RRID: AB_3742887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q92664
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTF3A encodes general transcription factor IIIA (TFIIIA) whose role in transcriptional regulation of 5S ribosomal DNA genes (encoding 5S ribosomal RNA (rRNA)) has been well characterized. TFIIIA comprises nine zinc finger (ZF) domains, each containing two invariant pairs of cysteines and histidines coordinating one Zn2+ ion (PMID: 36399538).