Tested Applications
| Positive WB detected in | HEK-293T cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human thyroid cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
27494-1-AP targets GTF3C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26666 Product name: Recombinant human GTF3C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 59-188 aa of BC020981 Sequence: GFEDSPDQRRLPPEQESLSRLEQPDLSSEMSKVSKPRASKPGRKRGGRTRKGPKRPQQPNPPSAPLVPGLLDQSNPLSTPMPKKRGRKSKAELLLLKLSKDLDRPESQSPKRPPEDFETPSGERPRRRAA Predict reactive species |
| Full Name | general transcription factor IIIC, polypeptide 2, beta 110kDa |
| Calculated Molecular Weight | 101 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC020981 |
| Gene Symbol | GTF3C2 |
| Gene ID (NCBI) | 2976 |
| RRID | AB_2880889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GTF3C2, also named as KIAA0011, is a 911 amino acid protein, which localizes in the nucleus. GTF3C2 is required for RNA polymerase III-mediated transcription. GTF3C2 is a component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. GTF3C2 may play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GTF3C2 antibody 27494-1-AP | Download protocol |
| IHC protocol for GTF3C2 antibody 27494-1-AP | Download protocol |
| WB protocol for GTF3C2 antibody 27494-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |





















