GTF3C2 Polyclonal antibody

GTF3C2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27494-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

TFIIIC110, TFIIIC BETA, TF3C-beta, TF3C beta, KIAA0011

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293T cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman thyroid cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27494-1-AP targets GTF3C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26666

Product name: Recombinant human GTF3C2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 59-188 aa of BC020981

Sequence: GFEDSPDQRRLPPEQESLSRLEQPDLSSEMSKVSKPRASKPGRKRGGRTRKGPKRPQQPNPPSAPLVPGLLDQSNPLSTPMPKKRGRKSKAELLLLKLSKDLDRPESQSPKRPPEDFETPSGERPRRRAA

Predict reactive species
Full Name general transcription factor IIIC, polypeptide 2, beta 110kDa
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC020981
Gene Symbol GTF3C2
Gene ID (NCBI) 2976
RRIDAB_2880889
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTF3C2, also named as KIAA0011, is a 911 amino acid protein, which localizes in the nucleus. GTF3C2 is required for RNA polymerase III-mediated transcription. GTF3C2 is a component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. GTF3C2 may play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.

Protocols

Product Specific Protocols
WB protocol for GTF3C2 antibody 27494-1-APDownload protocol
IHC protocol for GTF3C2 antibody 27494-1-APDownload protocol
IF protocol for GTF3C2 antibody 27494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Genet

Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder

Authors - Aiko Iwata-Otsubo
Loading...
||
New chat Able
正在加载,请稍候...