Tested Applications
| Positive WB detected in | HEK-293T cells, A549 cells, HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells |
| Positive IHC detected in | human thyroid cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27494-1-AP targets GTF3C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26666 Product name: Recombinant human GTF3C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 59-188 aa of BC020981 Sequence: GFEDSPDQRRLPPEQESLSRLEQPDLSSEMSKVSKPRASKPGRKRGGRTRKGPKRPQQPNPPSAPLVPGLLDQSNPLSTPMPKKRGRKSKAELLLLKLSKDLDRPESQSPKRPPEDFETPSGERPRRRAA Predict reactive species |
| Full Name | general transcription factor IIIC, polypeptide 2, beta 110kDa |
| Calculated Molecular Weight | 101 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC020981 |
| Gene Symbol | GTF3C2 |
| Gene ID (NCBI) | 2976 |
| RRID | AB_2880889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GTF3C2, also named as KIAA0011, is a 911 amino acid protein, which localizes in the nucleus. GTF3C2 is required for RNA polymerase III-mediated transcription. GTF3C2 is a component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. GTF3C2 may play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GTF3C2 antibody 27494-1-AP | Download protocol |
| IHC protocol for GTF3C2 antibody 27494-1-AP | Download protocol |
| WB protocol for GTF3C2 antibody 27494-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GTF3C2 Polyclonal antibody (Cat# 27494-1-AP) has 2 total citations. These appear in Journal of Clinical and Translational Hepatology (2025) and Human Genetics (2024). The cited research spans hepatocellular carcinoma (investigating GTF3C2's role in tumor proliferation via the USP21/MEK2/ERK pathway) and human genetics (studying biallelic GTF3C5 variants causing a multisystem developmental disorder). The cited application is Western Blot in human samples.
| Species | Application | Title |
|---|---|---|
J Clin Transl Hepatol GTF3C2 Promotes the Proliferation of Hepatocellular Carcinoma Cells through the USP21/MEK2/ERK1/2 Pathway | ||
Hum Genet Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder |
