GTF3C4 Polyclonal antibody, PBS Only

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• GTF3C4 Polyclonal antibody, PBS Only (17653-1-PBS)

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  WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

GTF3C4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 17653-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

17653-1-PBS targets GTF3C4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11898

  Product name: Recombinant human GTF3C4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 473-822 aa of BC094774

  Sequence: GSVRTHGIAVSPCGAYLAIITTEGMINGLHPVNKNYQVQFVTLKTFEEAAAQLLESSVQNLFKQVDLIDLVRWKILKDKHIPQFLQEALEKKIESSGVTYFWRFKLFLLRILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPTTGDAGGREPMEEKLLEIQGKIEAVEMHLTREHMKRVLGEVYLHTWITENTSIPTRGLCNFLMSDEEYDDRTARVLIGHISKKMNKQTFPEHCSLCKEILPFTDRKQAVCSNGHIWLRCFLTYQSCQSLIYRRCLLHDSIARHPAPEDPDWIKRLLQSPCPFCDSPVF

• Full Name: general transcription factor IIIC, polypeptide 4, 90kDa
• Calculated Molecular Weight: 822 aa, 92 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC094774
• Gene Symbol: GTF3C4
• Gene ID (NCBI): 9329
• RRID: AB_2115253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKN8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GTF3C4, also named as TFIIIC90, is essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. It has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3.