GTPBP8 Polyclonal antibody

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• GTPBP8 Polyclonal antibody (19813-1-AP)

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  IP experiment of A549 using 19813-1-AP

  IP result of anti-GTPBP8 (IP:19813-1-AP, 4ug; Detection:19813-1-AP 1:500) with A549 cells lysate 1840 ug.

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  IF Staining of U2OS using 19813-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using GTPBP8 antibody (19813-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 19813-1-AP

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GTPBP8 Polyclonal Antibody for IF/ICC, IP, ELISA

Cat No. 19813-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Synonyms

GTP binding protein 8, GTP-binding protein 8

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Tested Applications

• Positive IP detected in: A549 cells
• Positive IF/ICC detected in: U2OS cells, HepG2 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19813-1-AP targets GTPBP8 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13848

  Product name: Recombinant human GTPBP8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-171 aa of BC000003

  Sequence: WEGRMAAPGLRLGAGRLFEMPAVLERLSRYNSTSQAFAEVLRLPKQQLRKLLYPLQEVERFLAPYGRQDLHLRIFDPSPEDIARADNIFTATERNRIDYVSSAVRIDHAPDLPRPEVCFIGRSNVGKSSLIKALFSLAPEVEVRVSKKPGHTKKMNFFKVGKHFTAGGHAR

• Full Name: GTP-binding protein 8 (putative)
• Calculated Molecular Weight: 284 aa, 32 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC000003
• Gene Symbol: GTPBP8
• Gene ID (NCBI): 29083
• RRID: AB_3741969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3Z3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTPBP8 is a protein-coding gene encoding a GTP-binding protein that plays a critical role in mitochondrial ribosome (mitoribosome) biogenesis . It is the human homolog of the bacterial EngB family of GTPases, which are involved in ribosomal large subunit assembly. (PMID: 38969660)