GUCA1C Polyclonal antibody

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• GUCA1C Polyclonal antibody (21531-1-AP)

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  IHC staining of rat eye using 21531-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21531-1-AP (GUCA1C antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat eye using 21531-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21531-1-AP (GUCA1C antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GUCA1C Polyclonal Antibody for IHC, ELISA

Cat No. 21531-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, ELISA

Synonyms

GCAP 3, GCAP3, guanylate cyclase activator 1C, Guanylyl cyclase-activating protein 3

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Tested Applications

• Positive IHC detected in: rat eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21531-1-AP targets GUCA1C in IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16157

  Product name: Recombinant human GUCA1C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-209 aa of BC103993

  Sequence: MGNGKSIAGDQKAVPTQETHVWYRTFMMEYPSGLQTLHEFKTLLGLQGLNQKANKHIDQVYNTFDTNKDGFIDFLEFIAAVNLIVQEKMEQKLKWYFKLYDADGNGSIDKNELLDMFMAVQALNGQQTLSPEEFINLVFHKIDINNDGELTLEEFINGMAKDQDLLEIVYKSFDFSNVLRVICNGKQPDMETDSSKSPDKAGLGKVKMK

• Full Name: guanylate cyclase activator 1C
• Calculated Molecular Weight: 209 aa, 24 kDa
• GenBank Accession Number: BC103993
• Gene Symbol: GUCA1C
• Gene ID (NCBI): 9626
• RRID: AB_3742042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95843
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The guanylate cyclase-activating proteins (GCAPs), Ca2+-binding proteins of the calmodulin gene superfamily, function as regulators of photoreceptor guanylate cyclases (PMID: 15336959). Three different GCAPs (GCAP1, GCAP2, and GCAP3) are identified in vertebrate retina (PMID: 12596930). The cone-specific guanylate cyclase-activating protein 3 (GCAP3) has been shown to regulate the enzymatic activity of membrane-bound guanylate cyclases (GCs) in bovine and teleost fish photoreceptors, to an extent comparable to that of the paralog protein GCAP1 (PMID: 35328663).