GUCY1A3 Polyclonal antibody

GUCY1A3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12605-1-AP

Synonyms

GC SA3, GCS alpha 1, GCS alpha 3, GUC1A3, GUCA3, GUCSA3, GUCY1A1, GUCY1A3



Tested Applications

Positive WB detected inhuman brain tissue, human cerebellum tissue, human kidney tissue, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12605-1-AP targets GUCY1A3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GUCY1A3 fusion protein Ag3293
Full Name guanylate cyclase 1, soluble, alpha 3
Calculated molecular weight 690 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC028384
Gene symbol GUCY1A3
Gene ID (NCBI) 2982
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GUCY1A3 antibody 12605-1-APDownload protocol
IHC protocol for GUCY1A3 antibody 12605-1-APDownload protocol
IP protocol for GUCY1A3 antibody 12605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Sci Rep

Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.

Authors - Catarina Rippe