GUCY2D Polyclonal antibody

GUCY2D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55127-1-AP

Synonyms

CORD5, CORD6, CYGD, Guanylate cyclase 2D, retinal, GUC1A4, GUC2D, GUCY2D, LCA, LCA1, retGC, RETGC 1, RETGC1, Retinal guanylyl cyclase 1, ROS GC, ROS GC1

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Validation Data Gallery

• 

  WB analysis of mouse brain using 55127-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55127-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55127-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

55127-1-AP targets GUCY2D in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: guanylate cyclase 2D, membrane (retina-specific)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa, 95 kDa
• GenBank Accession Number: NM_000180
• Gene Symbol: GUCY2D
• Gene ID (NCBI): 3000
• RRID: AB_10804281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GUCY2D, also named as CORD6, GUC1A4, GUC2D, RETGC, RETGC1 and ROS-GC, belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. A number of nonsense and frameshift mutations in the GUCY2D gene have been identified in LCA1 patients. Defects in GUCY2D are the cause of cone-rod dystrophy type 6 (CORD6). GUCY2D is responsible for many reported cases of autosomal dominant CRDs. The antibody is specific to GUCY2D.

Publications

Species	Application	Title
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta