GUK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GUK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10755-1-AP

Synonyms

GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1

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Validation Data Gallery

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  WB analysis of HEK-293 using 10755-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10755-1-AP

  WB result of GUK1 antibody (10755-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells.

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  WB analysis of HeLa using 10755-1-AP

  WB result of GUK1 antibody (10755-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 10755-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10755-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10755-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 10755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10755-1-AP targets GUK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GUK1 fusion protein Ag1205
• Full Name: guanylate kinase 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-27 kDa
• GenBank Accession Number: BC009914
• Gene Symbol: GUK1
• Gene ID (NCBI): 2987
• RRID: AB_2115968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.