GYG1 Polyclonal antibody

GYG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12836-1-AP

Synonyms

glycogenin 1, GN 1, GN1, GYG, GYG1

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Validation Data Gallery

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  WB analysis using 12836-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12836-1-AP (GYG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 12836-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12836-1-AP (GYG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 12836-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 12836-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 12836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 12836-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, human testis tissue, U-251 cells
• Positive IHC detected in: human thyroid cancer tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12836-1-AP targets GYG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GYG1 fusion protein Ag3583
• Full Name: glycogenin 1
• Calculated Molecular Weight: 350 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC031096
• Gene Symbol: GYG1
• Gene ID (NCBI): 2992
• RRID: AB_2116101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan