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GYPC Polyclonal antibody, PBS Only (18147-1-PBS)

Filter: AllWBIHCIF-PIF/ICC

WB analysis of human blood using 18147-1-AP (same clone as 18147-1-PBS)
human blood were subjected to SDS PAGE followed by western blot with 18147-1-AP (GYPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 18147-1-AP (same clone as 18147-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 18147-1-AP (same clone as 18147-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.
IF Staining of K-562 using 18147-1-AP (same clone as 18147-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GYPC antibody (18147-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

GYPC Polyclonal antibody, PBS Only

Name: GYPC Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 18147-1-PBS

GYPC Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 18147-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

18147-1-PBS targets GYPC in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12825 Product name: Recombinant human GYPC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC106051 Sequence: MWSTRSPNSTAWPLSLEPDPGMASASTTMHTTTIAEPDPGMSGWPDGRMETSTPTIMDIVVIAGVIAAVAIVLVSLLFVMLRYMYRHKGTYHTNEAKGTEFAESADAALQGDPALQDAGDSSRKEYFI Predict reactive species
Full Name	glycophorin C (Gerbich blood group)
Calculated Molecular Weight	128 aa, 14 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC106051
Gene Symbol	GYPC
Gene ID (NCBI)	2995
RRID	AB_2878508
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04921
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.