Galectin-1 Recombinant monoclonal antibody

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  WB analysis of NIH/3T3 using 85091-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85091-1-RR (Galectin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85091-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85091-1-RR (Galectin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85091-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85091-1-RR (Galectin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Galectin-1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85091-1-RR

Clone No.242313B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242313B10, Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1

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Tested Applications

• Positive WB detected in: HL-60 cells, NIH/3T3 cells, THP-1 cells, HeLa cells, A549 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85091-1-RR targets Galectin-1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2432

  Product name: Recombinant human Galectin 1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-135 aa of BC020675

  Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

• Full Name: lectin, galactoside-binding, soluble, 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC020675
• Gene Symbol: Galectin-1
• Gene ID (NCBI): 3956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P09382
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).