Galectin 10 Polyclonal antibody

Galectin 10 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25225-1-AP

Synonyms

Charcot Leyden crystal protein, CLC, Eosinophil lysophospholipase, Gal 10, Galectin 10, LGALS10, LPPL_HUMAN, Lysolecithin acylhydrolase



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25225-1-AP is Galectin 10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin 10 fusion protein Ag18569
Full Name Charcot-Leyden crystal protein
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC119711
Gene symbol CLC
Gene ID (NCBI) 1178
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Galectin 10 antibody 25225-1-APDownload protocol
IHC protocol for Galectin 10 antibody 25225-1-APDownload protocol
IF protocol for Galectin 10 antibody 25225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cells

CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.

Authors - Ssu-Ju Fu
humanIHC

Syst Biol Reprod Med

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Authors - Genhai Zhu