Galectin 10 Polyclonal antibody

Galectin 10 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 25225-1-AP

Synonyms

Charcot Leyden crystal protein, CLC, Eosinophil lysophospholipase, Gal 10, Galectin 10, LGALS10, LPPL_HUMAN, Lysolecithin acylhydrolase



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25225-1-AP is Galectin 10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin 10 fusion protein Ag18569
Full Name Charcot-Leyden crystal protein
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC119711
Gene symbol CLC
Gene ID (NCBI) 1178
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Galectin 10 antibody 25225-1-APDownload protocol
IHC protocol for Galectin 10 antibody 25225-1-APDownload protocol
IF protocol for Galectin 10 antibody 25225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Syst Biol Reprod Med

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Authors - Genhai Zhu
humanIF

Cells

CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.

Authors - Ssu-Ju Fu