Glucagon Monoclonal antibody

Glucagon Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse and More (1)







Cat no : 67286-1-Ig


GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

Tested Applications

Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas tissue, mouse pancreas tissue

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:2000-1:15000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 3 publications below

Product Information

67286-1-Ig targets Glucagon in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glucagon fusion protein Ag10629
Full Name glucagon
Calculated Molecular Weight 180 aa, 21 kDa
GenBank Accession NumberBC005278
Gene Symbol GCG
Gene ID (NCBI) 2641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.


Product Specific Protocols
IHC protocol for Glucagon antibody 67286-1-IgDownload protocol
IF protocol for Glucagon antibody 67286-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Food Chem X

Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.

Authors - Jingyu Fang

Mol Cell Endocrinol

Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.

Authors - Xinghong Guo

NPJ Biofilms Microbiomes

Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin

Authors - Manyun Chen

Mol Metab

Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function

Authors - Linlin Wang