CoraLite® Plus 488-conjugated Glucagon Monoclonal antibody

Glucagon Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

2A8G1

Cat no : CL488-67286

Synonyms

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

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Validation Data Gallery

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  IF Staining of mouse pancreas using CL488-67286

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-67286 (Glucagon antibody) and CL594-66198 (INS antibody), at dilution of 1:400. The B cells of the islets of Langerhans was labelled in red with CL594-66198(INS antibody).

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Tested Applications

• Positive IF detected in: mouse pancreas tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-67286 targets Glucagon in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glucagon fusion protein Ag10629
• Full Name: glucagon
• Calculated Molecular Weight: 180 aa, 21 kDa
• GenBank Accession Number: BC005278
• Gene Symbol: GCG
• Gene ID (NCBI): 2641
• RRID: AB_2883402
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon fromthe pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Publications

Species	Application	Title
mouse	IF	iScience; Optimal HSF1 activation in response to acute cold stress in BAT requires nuclear TXNIP; Authors - Althea N Waldhart