CoraLite®594-conjugated Glucagon Monoclonal antibody

Glucagon Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A8G1

Cat no : CL594-67286

Synonyms

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM



Tested Applications

Positive IF detected inmouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy Glucagon Ready-To-Use IHC Kit. Glucagon primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67286-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL594-67286 targets Glucagon in IF applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glucagon fusion protein Ag10629
Full Name glucagon
Calculated molecular weight 180 aa, 21 kDa
GenBank accession numberBC005278
Gene symbol GCG
Gene ID (NCBI) 2641
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon fromthe pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Protocols

Product Specific Protocols
IF protocol for CL594 Glucagon antibody CL594-67286Download protocol
Standard Protocols
Click here to view our Standard Protocols