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Tested Applications
| Positive WB detected in | Jurkat cells, TT cells, HuH-7 cells, mouse cerebellum tissue, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87773-1-RR targets Gpr44 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | G protein-coupled receptor 44 |
| Observed Molecular Weight | 35 kDa, 40 kDa |
| GenBank Accession Number | BC111906 |
| Gene Symbol | Gpr44 |
| Gene ID (NCBI) | 14764 |
| RRID | AB_3745746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Z2J6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPR44 (PTGDR2/CRTH2) is a Gi-coupled prostaglandin D₂ receptor expressed on Th2 lymphocytes, eosinophils, and basophils, where it mediates chemotaxis and activation of type 2 immune cells. Through PGD₂-dependent signaling, GPR44 plays a central role in allergic inflammation and eosinophilic immune responses, making it an important therapeutic target in asthma and other Th2-mediated diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Gpr44 antibody 87773-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
