H2AFY2 Antibody

H2AFY2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17030-1-AP

Synonyms

Core histone macro H2A.2, H2A histone family, member Y2, H2AFY2, Histone macroH2A2, macroH2A2, mH2A2



Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, mouse kidney tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

The 17030-1-AP immunogen is Fusion Protein H2AFY2 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen H2AFY2 fusion protein Ag10487
Full Name H2A histone family, member Y2
Calculated molecular weight 372aa,40 kDa
Observed molecular weight 40 kDa
GenBank accession number BC016172
Gene symbol H2AFY2
Gene ID (NCBI) 55506
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for H2AFY2 antibody 17030-1-APDownload protocol
IP protocol for H2AFY2 antibody 17030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Arch Biochem Biophys

ROR alpha protects against LPS-induced inflammation by down-regulating SIRT1/NF-kappa B pathway.

Authors - Shichao Han
mouseWB

J Cell Physiol

MCPIP1 alleviated lipopolysaccharide-induced liver injury by regulating SIRT1 via modulation of microRNA-9.

Authors - Shichao Han
mouseWB

Immunol Cell Biol

ING4 alleviated lipopolysaccharide-induced inflammation by regulating the NF-κB pathway via a direct interaction with SIRT1.

Authors - Yunshu Yang
mouseWB

Cell Physiol Biochem

Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.

Authors - Zhenzhen Li