H2AFY2 Polyclonal antibody

H2AFY2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17030-1-AP

Synonyms

Core histone macro H2A.2, H2A histone family, member Y2, H2AFY2, histone, Histone macroH2A2, macroH2A2, mH2A2

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 17030-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17030-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17030-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17030-1-AP

  IP result of anti-H2AFY2 (IP:17030-1-AP, 4ug; Detection:17030-1-AP 1:500) with HepG2 cells lysate 1000ug.

---

Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, mouse kidney tissue
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

17030-1-AP targets H2AFY2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: H2AFY2 fusion protein Ag10487
• Full Name: H2A histone family, member Y2
• Calculated Molecular Weight: 372 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC016172
• Gene Symbol: H2AFY2
• Gene ID (NCBI): 55506
• RRID: AB_10638483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.; Authors - Zhenzhen Li
mouse	WB	Cell Physiol Biochem; Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.; Authors - Zhenzhen Li
mouse	WB	Immunol Cell Biol; ING4 alleviated lipopolysaccharide-induced inflammation by regulating the NF-κB pathway via a direct interaction with SIRT1.; Authors - Yunshu Yang
mouse	WB	Arch Biochem Biophys; ROR alpha protects against LPS-induced inflammation by down-regulating SIRT1/NF-kappa B pathway.; Authors - Shichao Han
mouse	WB	J Cell Physiol; MCPIP1 alleviated lipopolysaccharide-induced liver injury by regulating SIRT1 via modulation of microRNA-9.; Authors - Shichao Han