Histone H3.3 Polyclonal antibody

Histone H3.3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13754-1-AP

Synonyms

H3 histone, family 3A, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, Histone H3.3



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, mouse thymus tissue, mouse kidney tissue, mouse lung tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13754-1-AP targets Histone H3.3 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H3.3 fusion protein Ag4696
Full Name H3 histone, family 3A
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC038989
Gene symbol H3F3A
Gene ID (NCBI) 3020
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].

Protocols

Product Specific Protocols
WB protocol for Histone H3.3 antibody 13754-1-APDownload protocol
IHC protocol for Histone H3.3 antibody 13754-1-APDownload protocol
IF protocol for Histone H3.3 antibody 13754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

ADAM10 Plasma and CSF Levels Are Increased in Mild Alzheimer's Disease.

Authors - Izabela Pereira Vatanabe