ChromoTek HA Monoclonal antibody (7C9)

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

Specificity

Spot-Tag

Applications

IF, WB

Conjugate

0.5 – 1 mg/mL (conjugates)

Cat no : 7c9

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Label



Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

ApplicationsIF, WB
Recommended DilutionIF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag (PDRVRAVSHWSS)
GeneID (NCBI)0
Conjugate0.5 – 1 mg/mL (conjugates)
Storage BufferBuffered aqueous solution (PBS)
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C / 40°F.Stable for 6 months. Do not freeze. Protect from light.

Publications

ApplicationTitle
WB

Cell Rep

The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity.

Authors - Torsten Schultz-Larsen
IF

Life Sci Alliance

Oxidised metabolites of the omega-6 fatty acid linoleic acid activate dFOXO.

Authors - So Yeon Kwon
WB

Traffic

Vaccinia virus proteins A36 and F12/E2 show strong preferences for different kinesin light chain isoforms.

Authors - William N D Gao
WB

J Biol Chem

TNF receptor-associated factor 6 interacts with ALS-linked misfolded superoxide dismutase 1 and promotes aggregation.

Authors - Sabrina Semmler
IP

J Biol Chem

The secreted protein kinase CstK from Coxiella burnetii influences vacuole development and interacts with the GTPase-activating host protein TBC1D5.

Authors - Eric Martinez
IHC

Elife

Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery.

Authors - Ryan T Dosumu-Johnson