ChromoTek HA Monoclonal antibody (7C9)
anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins
Specificity
Spot-Tag
Applications
IF, WB
Conjugate
0.5 – 1 mg/mL (conjugates)
Cat no : 7c9
Synonyms
Validation Data Gallery
Product Information
anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins
| Applications | IF, WB |
| Recommended Dilution | IF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 6 nM |
| Specificity/Target | Spot-Tag (PDRVRAVSHWSS) |
| GeneID (NCBI) | 0 |
| Conjugate | 0.5 – 1 mg/mL (conjugates) |
| Storage Buffer | Buffered aqueous solution (PBS) |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C / 40°F.Stable for 6 months. Do not freeze. Protect from light. |
Documentation
| SDS |
|---|
| SDS Spot-Label ATTO488 |
| Protocol |
|---|
| Protocol Spot-Label ATTO488 |
| Brochure |
|---|
| Product brochure (PDF) |
Publications
| Application | Title |
|---|---|
Life Sci Alliance Oxidised metabolites of the omega-6 fatty acid linoleic acid activate dFOXO. | |
Traffic Vaccinia virus proteins A36 and F12/E2 show strong preferences for different kinesin light chain isoforms. | |
J Biol Chem TNF receptor-associated factor 6 interacts with ALS-linked misfolded superoxide dismutase 1 and promotes aggregation. | |
J Biol Chem The secreted protein kinase CstK from Coxiella burnetii influences vacuole development and interacts with the GTPase-activating host protein TBC1D5. | |
Elife Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery. |