Validation Data Gallery
Product Information
Rat monoclonal [7C9] to HA-tag
| Description | HA-tag rat monoclonal [7C9] for WB, IF, IP |
| Applications | WB, IF, IP |
| Specificity/Target | HA-tag (YPYDVPDYA) at the N-terminus, C-terminus, or internal site of fusion protein. |
| Recommended Dilution | Western Blot: 1:1,000 Immunofluorescence: 1:1,000 Immunoprecipitation: 5 µg/ IP |
| Host/IsoType | Rat/IgG2a |
| Class | Monoclonal |
| Type | Primary Antibody |
| Clone No. | 7C9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purified |
| RRID | AB_2631399 |
| Storage Buffer | PBS, preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment. |
| Size | 20 µl, 100 µl |
Documentation
| SDS |
|---|
| 7c9_SDS_rat anti HA Monoclonal antibody (7C9) (EN) |
| Datasheet |
|---|
| rat anti HA Monoclonal antibody (7C9) Datasheet |
Publications
| Application | Title |
|---|---|
Nat Commun Maturation and substrate processing topography of the Plasmodium falciparum invasion/egress protease plasmepsin X | |
Pharmacol Res RHBDF1 deficiency suppresses melanoma glycolysis and enhances efficacy of immunotherapy by facilitating glucose-6-phosphate isomerase degradation via TRIM32 | |
New Phytol The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH William (Verified Customer) (09-06-2025) | The antibody was used to label HA in human iPSC-derived cardiomyocytes transduced with AAV6-HA-tagged Actinin by ICC. It worked well. But, note strong nucleus staining; It could be attributable to either bleed-through from nucleus labeling with DAPI or the immaturity of the human iPSC-derived cells.
 |
FH kaihua (Verified Customer) (04-17-2025) | Worked very nice at 1:500 dilution on mouse liver tissue (violet staining in the picture). Did not have cross reactivity with other antibodies that were co-stained in this experiment.
 |
FH Tsimafei (Verified Customer) (12-04-2022) | Cells expressing HA-GFP were subjected to IP and the membrane was probed with rat anti-HA (7C9) antibody. Great performance of the antibody!
 |
