Filter: AllWBIF/ICCAffinity

WB analysis using 86673-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86673-1-RR (HAAO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 86673-1-RR
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HAAO antibody (86673-1-RR, Clone: 251701F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Affinity and Kinetic Characterization of 86673-1-RR
Biolayer interferometry (BLl) kinetic assays of 86673-1-RR against Human HAAO were performed. The affinity constant is 8.17 nM.

HAAO Recombinant monoclonal antibody

HAAO Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86673-1-RR

Clone No.251701F11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

3 HAO, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid dioxygenase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse liver tissue, rat liver tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86673-1-RR targets HAAO in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag3511 Product name: Recombinant human HAAO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC029510 Sequence: MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG Predict reactive species
Full Name	3-hydroxyanthranilate 3,4-dioxygenase
Calculated Molecular Weight	286 aa, 33 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC029510
Gene Symbol	HAAO
Gene ID (NCBI)	23498
RRID	AB_3745045
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P46952
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAAO is also known by several synonyms, including 3-HAO (3-hydroxyanthranilate oxygenase), HAO (3-hydroxyanthranilic acid dioxygenase), h3HAO and HAD. HAAO is widely distributed in peripheral organs, with particularly high expression in the liver and kidney. Strong positivity has been observed in the cytoplasm of hepatocytes and renal tubules, as well as in cilia of respiratory epithelium and fallopian tube epithelium. Moderate staining is detected in the small intestine and subsets of lymphoid cells.